电离辐射通过转化生长因子-β-介导的上皮-间质匹配来促进癌细胞的侵袭迁移

Int J Radiat Oncol Biol Phys 2011 Dec;81 (5): 1530-7. [IF:4.503]Ionizing radiation promotes migration and invasion of cancer cells through transforming growth factor-Beta-mediated epithelial-mesenchymal transition.Zhou YC , Liu JY , Li J , Zhang J , Xu YQ , Zhang HW , Qiu LB , Ding GR , Su XM , Mei-Shi , Guo GZ .Department of Radiation Oncology, Xijing Hospital Fourth Military Medical University, Xi'an, China; Department of Radiation Medicine, College of Preventive Medicine, Xijing Hospital Fourth Military Medical University, Xi'an, China.第四军医大学西京医院放射科

AbstractTo examine whether ionizing radiation enhances the migratory and invasive abilities of cancer cells through transforming growth factor (TGF-β)-mediated epithelial-mesenchymal transition (EMT). Six cancer cell lines originating from different human organs were irradiated by (60)Co γ-ray at a total dose of 2 Gy, and the changes associated with EMT, including morphology, EMT markers, migration and invasion, were observed by microscope, Western blot, immunofluorescence, scratch assay, and transwell chamber assay, respectively. Then the protein levels of TGF-β in these cancer cells were detected by enzyme-linked immunosorbent assay, and the role of TGF-β signaling pathway in the effect of ionizing radiation on EMT was investigate by using the specific inhibitor SB431542. After irradiation with γ-ray at a total dose of 2 Gy, cancer cells presented the mesenchymal phenotype, and compared with the sham-irradiation group the expression of epithelial markers was decreased and of mesenchymal markers was increased, the migratory and invasive capabilities were strengthened, and the protein levels of TGF-β were enhanced. Furthermore, events associated with EMT induced by IR in A549 could be reversed through inhibition of TGF-β signaling. These results suggest that EMT mediated by TGF-β plays a critical role in IR-induced enhancing of migratory and invasive capabilities in cancer cells.

简短 ：探讨辐射源是否可通过转化介素-β（TGF-β）-介导的角质层-脂质切换 (EMT)来作出贡献恶性肿瘤的侵扰迁到。使用据统计2Gy(60)Coγ线照射来源于人类器官的6种恶性肿瘤，据信与EMT相关的变化，这包括分别利用电子显微镜关键技术，受体质印迹方律，免疫荧光关键技术，划痕试验和Transwell小室试验来推论并检测细胞组织型态，EMT标记，侵扰迁到并能等。采用酶联免疫吸附律检测这些恶性肿瘤之前TGF-β受体程度，利用除此以外诱发剂SB431542来评估TGF-β信号通路在辐射源EMT之前的作用。经过据统计为2Gy照射的恶性肿瘤之前存在间叶细胞的表述，与假照射组相比之下其角质层标记减少，间叶细胞标记增加，同时其侵扰集中于并能增强，TGF-β受体程度也提高。进一步发现由A549辐射源诱导的EMT可通过对TGF-β信号诱发发生逆转。这些结果表明TGF-β介导的EMT在辐射源诱导增强恶性肿瘤侵扰集中于并能之前起着不可忽视。

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